ʻIke Hou no ka Platelet Rich Plasma (PRP) Therapy – Māhele I

Hiki ke hoʻohana i ka lāʻau lapaʻau autologous e hoʻohana ana i ka platelet-rich plasma (PRP) i mea kōkua i nā hoʻolālā lāʻau lapaʻau regenerative.Loaʻa kahi koi honua ʻole no nā hoʻolālā hoʻoponopono kikoʻī no ka mālama ʻana i nā maʻi me ka musculoskeletal (MSK) a me nā maʻi spinal, osteoarthritis (OA) a me nā ʻeha paʻakikī a me ka refractory.Hoʻokumu ʻia ka lāʻau lapaʻau PRP ma ka ʻoiaʻiʻo ʻo ka platelet growth factor (PGF) e kākoʻo i ka ho'ōla ʻana i ka ʻeha a me ka hoʻoponopono ʻana i ka cascade (inflammation, proliferation and remodeling).Ua loiloi ʻia kekahi mau ʻano hana PRP mai ke kanaka, in vitro a me nā haʻawina holoholona.Eia nō naʻe, ke alakaʻi pinepine ʻia nā ʻōlelo aʻoaʻo o ka in vitro a me nā haʻawina holoholona i nā hopena lapaʻau like ʻole, no ka mea paʻakikī ke unuhi i nā hopena noiʻi non-clinical a me nā ʻōlelo aʻoaʻo i loko o ka lāʻau lapaʻau kanaka.I nā makahiki i hala iho nei, ua holomua ka hoʻomaopopo ʻana i ka manaʻo o ka ʻenehana PRP a me nā mea ola olaola, a ua noi ʻia nā ʻōlelo noiʻi hou a me nā hōʻailona hou.Ma kēia loiloi, e kūkākūkā mākou i ka holomua hou loa i ka hoʻomākaukau ʻana a me ka hoʻohui ʻana o PRP, e komo pū me ka platelet dose, leukocyte activity a me ka hoʻoponopono kūlohelohe kūlohelohe a me ka adaptive immune, 5-hydroxytryptamine (5-HT) hopena a me ka hōʻeha ʻeha.Eia kekahi, ua kūkākūkā mākou i ka mīkini PRP e pili ana i ka mumū a me ka angiogenesis i ka wā o ka hoʻoponopono ʻana a me ka hana hou.ʻO ka hope, e nānā mākou i nā hopena o kekahi mau lāʻau i ka hana PRP.

ʻO ka autologous platelet-rich plasma (PRP) ka ʻāpana wai o ke koko peripheral autologous ma hope o ka mālama ʻana, a ʻoi aku ka kiʻekiʻe o ka platelet ma mua o ka palena.Ua hoʻohana ʻia ka lāʻau PRP no nā hōʻailona like ʻole ma mua o 30 mau makahiki, e hopena i ka hoihoi nui i ka hiki o ka autogenous PRP i ka lāʻau lapaʻau regenerative.Ua hoʻolauna ʻia ka huaʻōlelo orthopedic biological agent no ka mālama ʻana i nā maʻi musculoskeletal (MSK), a ua hoʻokō i nā hopena hoʻohiki i ka hiki ke hoʻolaʻa hou i nā hui pūlima PRP bioactive heterogeneous.I kēia manawa, ʻo ka PRP therapy kahi koho lapaʻau kūpono me nā pōmaikaʻi lapaʻau, a ke paipai nei nā hopena maʻi i hōʻike ʻia.Eia nō naʻe, ʻo ka like ʻole o nā hopena maʻi a me nā ʻike hou i hoʻopilikia i ka hiki ke hoʻohana ʻia o ka PRP.ʻO kekahi o nā kumu ka helu a me ka loli o nā ʻōnaehana PRP a me PRP-type ma ka mākeke.He ʻokoʻa kēia mau mea hana ma ke ʻano o ka nui o ka hōʻiliʻili PRP a me ka hoʻomākaukau ʻana, e hopena i nā hiʻohiʻona PRP kūʻokoʻa a me nā mea ola.Eia kekahi, ʻo ka nele o ka ʻae ʻana i ka standardization o ka papahana hoʻomākaukau PRP a me ka hōʻike piha o nā mea ola ola i ka noi ʻana i alakaʻi i nā hopena hōʻike like ʻole.Nui nā hoʻāʻo ʻana e ʻike a hoʻokaʻawale i ka PRP a i ʻole nā ​​​​huahana i loaʻa i ke koko i nā noi lāʻau lapaʻau hou.Eia kekahi, ua noi ʻia nā derivatives platelet, e like me nā platelet lysates kanaka, no ka noiʻi orthopedic a in vitro stem cell.

Ua paʻi ʻia kekahi o nā manaʻo mua e pili ana i ka PRP i ka makahiki 2006. ʻO ka manaʻo nui o kēia loiloi ka hana a me ke ʻano o ka hana o nā platelets, ka hopena o PRP ma kēlā me kēia pae o ka cascade ho'ōla, a me ke kuleana nui o ka platelet-derived growth factor. i nā hōʻailona PRP like ʻole.I ka hoʻomaka ʻana o ka noiʻi PRP, ʻo ka makemake nui i ka PRP a i ʻole PRP-gel ʻo ke ola a me nā hana kikoʻī o kekahi mau platelet growth factor (PGF).

Ma kēia pepa, e kūkākūkā nui mākou i ka hoʻomohala hou ʻana o nā ʻano ʻāpana PRP like ʻole a me nā mea hoʻopaʻa membrane cell platelet a me kā lākou hopena i ka hoʻoponopono ʻana i ka ʻōnaehana immune a me ka adaptive.Eia kekahi, e kūkākūkā nui ʻia ke kuleana o kēlā me kēia cell i loko o ka vial lapaʻau PRP a me kā lākou hopena i ke kaʻina hana hou.Eia kekahi, e wehewehe ʻia ka holomua hou loa i ka hoʻomaopopo ʻana i nā mea ola ola PRP, platelet dose, nā hopena kikoʻī o nā keʻokeʻo keʻokeʻo kikoʻī, a me nā hopena o ka manaʻo o ka PGF a me nā cytokines i nā hopena meaʻai o nā mesenchymal stem cell (MSCs) e wehewehe ʻia, me ka PRP e huli ana i nā ʻano like ʻole. kaiapuni a me ka ʻiʻo ma hope o ka lawe ʻana i ka hōʻailona cell a me nā hopena paracrine.Pēlā nō, e kūkākūkā mākou i ka mīkini PRP e pili ana i ka mumū a me ka angiogenesis i ka wā o ka hoʻoponopono ʻana a me ka hana hou.ʻO ka hope, e nānā mākou i ka hopena analgesic o PRP, ka hopena o kekahi mau lāʻau i ka hana PRP, a me ka hui pū ʻana o PRP a me nā papahana hoʻoponopono.

Nā loina kumu o ka lāʻau lapaʻau plasma waiwai nui o ka platelet

Ua kaulana a hoʻohana nui ʻia nā hoʻomākaukau PRP ma nā ʻano lāʻau lapaʻau.ʻO ke kumu ʻepekema kumu o ka mālama ʻana i ka PRP ʻo ia ka hoʻomaka ʻana o nā platelets concentrated ma ka wahi i hōʻeha ʻia e hoʻomaka i ka hoʻoponopono ʻana i ka ʻiʻo, ka synthesis o ka ʻiʻo pili hou a me ke kūkulu hou ʻana o ke kahe koko ma o ka hoʻokuʻu ʻana i nā mea he nui biologically active (growth factor, cytokines, lysosomes) a nā protein adhesion ke kuleana no ka hoʻomaka ʻana i ka hopena cascade hemostatic.Eia kekahi, aia nā protein plasma (e laʻa, fibrinogen, prothrombin, a me fibronectin) i loko o nā mea platelet-poor plasma parts (PPPs).Hiki i ka PRP concentrate ke hoʻoulu i ka hoʻokuʻu hyperphysiological o nā mea ulu e hoʻomaka ai i ka ho'ōla ʻana i ka ʻeha mau a me ka wikiwiki i ke kaʻina hana hoʻoponopono o ka hōʻeha ʻeha.Ma nā pae āpau o ke kaʻina hana hoʻoponopono kino, hoʻolaha nā ʻano mea ulu like ʻole, cytokines a me nā mea hoʻoponopono hana kūloko i ka hapa nui o nā hana cell ma o endocrine, paracrine, autocrine a me nā hana endocrine.ʻO nā pōmaikaʻi nui o ka PRP e pili ana i kona palekana a me ka ʻenehana hoʻomākaukau noʻonoʻo o nā lako kalepa o kēia manawa, hiki ke hoʻohana ʻia no ka hoʻomākaukau ʻana i nā mea olaola hiki ke hoʻohana nui ʻia.ʻO ka mea nui loa, ke hoʻohālikelike ʻia me nā corticosteroids maʻamau, ʻo PRP kahi huahana autogenous me ka ʻike ʻole ʻia o nā hopena ʻaoʻao.Eia nō naʻe, ʻaʻohe hoʻoponopono maopopo i ke ʻano a me ka haku mele ʻana o ka haku mele PRP injectable, a ʻo ka haku ʻana o ka PRP he mau loli nui i nā platelets, ka maʻiʻo keʻokeʻo keʻokeʻo (WBC), ka pollution o ke koko ʻulaʻula (RBC), a me ka ʻike PGF.

PRP hua'ōlelo a me ka hoʻokaʻawale

No nā makahiki he nui, ʻo ka hoʻomohala ʻana o nā huahana PRP i hoʻohana ʻia e hoʻoulu i ka hoʻoponopono ʻana a me ka hana hou ʻana he wahi noiʻi koʻikoʻi o nā biomaterials a me ka ʻepekema lāʻau.ʻO ka cascade healing tissue ka nui o nā mea komo, me nā platelets a me kā lākou ulu ulu a me nā cytokine granules, nā keʻokeʻo koko keʻokeʻo, fibrin matrix a me nā cytokines synergistic he nui.Ma kēia kaʻina hana cascade, e hana ʻia kahi kaʻina coagulation paʻakikī, me ka hoʻoulu ʻana o ka platelet a me ka densification hope a me ka α- Ka hoʻokuʻu ʻana o nā mea i loko o nā ʻāpana platelet, ka hoʻohui ʻana o fibrinogen (i hoʻokuʻu ʻia e nā platelets a manuahi paha i loko o ka plasma) i loko o ka pūnaewele fibrin, a me ka hoʻokumu ʻana. o ka platelet embolism.

Hoʻohālikelike ka "Universal" PRP i ka hoʻomaka o ka ho'ōla

I ka wā mua, ua kapa ʻia ka huaʻōlelo "platelet-rich plasma (PRP)" i hoʻohana ʻia i ka lāʻau lapaʻau hoʻokahe koko, a ke hoʻohana ʻia nei i kēia lā.I ka hoʻomaka ʻana, ua hoʻohana wale ʻia kēia mau huahana PRP e like me ka fibrin tissue adhesive, ʻoiai ua hoʻohana wale ʻia nā platelets e kākoʻo i ka polymerization fibrin ikaika e hoʻomaikaʻi ai i ka hoʻopaʻa ʻana o ka ʻiʻo, ma mua o ka hoʻōla hoʻōla.Ma hope o kēlā, ua hoʻolālā ʻia ka ʻenehana PRP e hoʻohālikelike i ka hoʻomaka ʻana o ka cascade ho'ōla.Ma hope mai, ua hōʻuluʻulu ʻia ka ʻenehana PRP ma o kona hiki ke hoʻolauna a hoʻokuʻu i nā mea ulu i loko o ka microenvironment kūloko.ʻO kēia hoihoi no ka lawe ʻana i ka PGF e hūnā pinepine i ka hana koʻikoʻi o nā mea ʻē aʻe i loko o kēia mau mea koko.Hoʻonui hou ʻia kēia hoihoi ma muli o ka nele o ka ʻikepili ʻepekema, nā manaʻo mystical, nā makemake pāʻoihana a me ka nele o ka standardization a me ka hoʻokaʻawale ʻana.

He paʻakikī ka biology o ka PRP concentrate e like me ke koko ponoʻī, a ʻoi aku ka paʻakikī ma mua o nā lāʻau lapaʻau kahiko.ʻO nā huahana PRP nā mea ola ola.ʻO nā hopena o ka noi PRP lāʻau lapaʻau e pili ana i nā hiʻohiʻona intrinsic, universal a adaptive o ke koko o ka mea maʻi, me nā ʻāpana kelepona ʻē aʻe i hiki ke noho i loko o ka laʻana PRP a me ka microenvironment kūloko o ka mea loaʻa, hiki ke loaʻa i ke kūlana koʻikoʻi a maʻi paha.

Ka hōʻuluʻulu o ka huikau PRP hua'ōlelo a me ka manaʻo hoʻokaʻawale ʻōnaehana

No nā makahiki he nui, ua hoʻopilikia ʻia ka poʻe loea, ʻepekema a me nā ʻoihana e ka hoʻomaopopo mua ʻana a me nā hemahema o nā huahana PRP a me kā lākou mau ʻōlelo like ʻole.Ua wehewehe kekahi mau mea kākau i ka PRP ma ke ʻano he platelet-wale nō, a ʻo nā mea ʻē aʻe i hōʻike ʻia aia pū kekahi i ka PRP i loko o nā keʻena koko ʻulaʻula, nā ʻano keʻokeʻo keʻokeʻo like ʻole, fibrin a me nā protein bioactive me ka hoʻonui ʻana.No laila, ua hoʻokomo ʻia ka nui o nā mea ola ola PRP i loko o ka hana lapaʻau.He mea hoʻohaʻahaʻa ka nele i ka wehewehe kikoʻī ʻana o nā mea ola.ʻO ka hāʻule ʻole o ka hoʻomākaukau ʻana i ka huahana a me ka hoʻomohala ʻana i ka ʻōnaehana hoʻohālikelike i alakaʻi i ka hoʻohana ʻana i ka nui o nā huahana PRP i wehewehe ʻia e nā huaʻōlelo a me nā pōkole.ʻAʻole ia he mea kupanaha ka hoʻololi ʻana i nā hoʻomākaukau PRP e alakaʻi i nā hopena maʻi like ʻole.

Ua hoʻohana mua ʻo Kingsley i ka huaʻōlelo "platelet-rich plasma" ma 1954. I nā makahiki he nui ma hope, ʻo Ehrenfest et al.Ua manaʻo ʻia ka ʻōnaehana hoʻonohonoho mua e pili ana i ʻekolu mau ʻano nui (platelet, leukocyte a me fibrin content), a ua māhele ʻia nā huahana PRP he ʻehā: P-PRP, LR-PRP, pure platelet-rich fibrin (P-PRF) a me leucocyte waiwai PRF (L-PRF).Hoʻomākaukau ʻia kēia mau huahana e ka ʻōnaehana pani piha piha a i ʻole ka protocol manual.I kēia manawa, ʻo Everts et al.ʻO ke koʻikoʻi o ka haʻi ʻana i nā keʻokeʻo keʻokeʻo i ka hoʻomākaukau ʻana o PRP.Manaʻo pū lākou i ka hoʻohana ʻana i nā huaʻōlelo kūpono e hōʻike i nā ʻano hana ʻole a i ʻole nā ​​​​ʻano hana o ka hoʻomākaukau PRP a me ka platelet gel.

ʻO Delong et al.ua hoʻolālā ʻia kahi ʻōnaehana helu PRP i kapa ʻia ʻo platelets, activated white blood cell (PAW) ma muli o ka helu piha o nā platelets, me nā pae ʻehā platelet.ʻO nā ʻāpana ʻē aʻe e pili ana i ka hoʻohana ʻana i nā platelet activators a me ka hele ʻana a i ʻole ka ʻole o nā keʻokeʻo keʻokeʻo (ʻo ia hoʻi nā neutrophils).Mishra et al.Hāʻawi ʻia kahi ʻōnaehana hoʻonohonoho like.I kekahi mau makahiki ma hope mai, ua wehewehe ʻo Mautner a me kāna mau hoa hana i kahi ʻōnaehana hoʻonohonoho kikoʻī a kikoʻī (PLRA).Ua hōʻoia ka mea kākau he mea nui ka wehewehe ʻana i ka helu platelet holoʻokoʻa, ka maʻi o ke koko keʻokeʻo (maikaʻi a maikaʻi ʻole paha), ka pakeneka neutrophil, RBC (maikaʻi a maikaʻi ʻole paha) a inā hoʻohana ʻia ka exogenous activation.Ma 2016, Magalon et al.Ua paʻi ʻia ka hoʻohālikelike ʻana o ka DEPA e pili ana i ka nui o ka platelet injection, ka hana pono, ka maʻemaʻe o ka PRP i loaʻa a me ke kaʻina hana.Ma hope mai, ua hoʻolauna ʻo Lana a me kāna mau hoa hana i ka ʻōnaehana hoʻokaʻawale MARSPILL, e kālele ana i nā cell mononuclear blood peripheral.I kēia mau lā, ua kākoʻo ke Kōmike Standardization Scientific i ka hoʻohana ʻana i ka ʻōnaehana papa o ka International Society for Thrombosis and Hemostasis, kahi i hoʻokumu ʻia ma ke ʻano o nā ʻōlelo aʻoaʻo e hoʻohālikelike i ka hoʻohana ʻana i nā huahana platelet i nā noi lāʻau lapaʻau regenerative, me nā huahana platelet frozen a thawed.

Ma muli o ka ʻōnaehana hoʻohālikelike PRP i manaʻo ʻia e nā loea a me nā mea noiʻi like ʻole, nui nā hoʻāʻo kūleʻa ʻole e hoʻohālikelike i ka hana, wehewehe a me ke ʻano o ka PRP e hoʻohana ʻia e nā kauka hiki ke huki i kahi hopena kūpono, ʻaʻole paha e hiki i nā makahiki e hiki mai ana. , Ke hoʻomau nei ka hoʻomohala ʻana o ka ʻenehana o nā huahana PRP clinical, a hōʻike ka ʻike ʻepekema e pono ai nā hoʻomākaukau PRP like ʻole e mālama i nā pathologies like ʻole ma lalo o nā kūlana kikoʻī.No laila, ke manaʻo nei mākou e hoʻomau ka ulu ʻana o nā ʻāpana a me nā loli o ka hana PRP kūpono i ka wā e hiki mai ana.

Ke holomua nei ke ala hoʻomākaukau PRP

Wahi a ka PRP terminology a me ka wehewehe ʻana i ka huahana, ua hoʻokuʻu ʻia nā ʻōnaehana hoʻohālikelike no nā ʻano PRP like ʻole.ʻO ka mea pōʻino, ʻaʻohe manaʻo e pili ana i ka ʻōnaehana hoʻokaʻawale piha o PRP a i ʻole nā ​​​​mea koko autologous a me nā huahana koko.ʻO ke kūpono, pono e nānā ka ʻōnaehana hoʻonohonoho i nā ʻano PRP like ʻole, nā wehewehe a me nā nomenclature kūpono e pili ana i nā hoʻoholo lapaʻau o nā maʻi me nā maʻi kūikawā.I kēia manawa, hoʻokaʻawale nā ​​noi orthopedic i ka PRP i ʻekolu mau ʻāpana: fibrin waiwai nui platelet (P-PRF), PRP waiwai leucocyte (LR-PRP) a me PRP deficient leucocyte (LP-PRP).ʻOiai ʻoi aku ka kikoʻī ma mua o ka wehewehe ʻana o ka huahana PRP maʻamau, ʻo LR-PRP a me LP-PRP nā kāʻei i ʻike ʻole i kahi kikoʻī i loko o ka ʻike koko keʻokeʻo.Ma muli o kāna mau ʻōnaehana pale a me nā mea hoʻokipa, ua hoʻopilikia nui nā keʻokeʻo keʻokeʻo i ka biology intrinsic o nā maʻi maʻi maʻi.No laila, hiki i nā mea olaola PRP i loaʻa i nā keʻokeʻo keʻokeʻo kikoʻī hiki ke hoʻoikaika nui i ka hoʻoponopono immune a me ka hoʻoponopono a me ka hoʻoponopono hou.ʻOi aku ka kikoʻī, nui nā lymphocytes i ka PRP, e hana ana i ka mea ulu e like me ka insulin a me ke kākoʻo ʻana i ka hoʻoponopono hou ʻana.

He kuleana koʻikoʻi nā monocytes a me nā macrophages i ke kaʻina hana o ka hoʻoponopono ʻana a me ke ʻano o ka hoʻoponopono ʻana i nā kiko.ʻAʻole maopopo ka nui o nā neutrophils i ka PRP.Ua hoʻoholo ʻia ʻo LP-PRP ma ke ʻano he hoʻomākaukau mua PRP ma o ka loiloi ʻōnaehana e hoʻokō i nā hopena lapaʻau kūpono o ka OA hui.Eia naʻe, ʻo Lana et al.Ke kū'ē nei ka hoʻohana ʻana o LP-PRP i ka mālama ʻana i ke kuli OA, e hōʻike ana he mea koʻikoʻi nā keʻokeʻo keʻokeʻo kikoʻī i ke kaʻina hana inflammatory ma mua o ka hoʻoulu hou ʻana o ka ʻiʻo, no ka mea, hoʻokuʻu lākou i nā molekele pro-inflammatory a me anti-inflammatory.Ua ʻike lākou ʻoi aku ka maikaʻi o ka hui ʻana o nā neutrophils a me nā platelets i hoʻāla ʻia ma mua o nā hopena maikaʻi ʻole i ka hoʻoponopono ʻana i nā kiko.Ua kuhikuhi pū lākou he mea nui ka plasticity o nā monocytes no ka hana non-inflammatory a me ka hoʻoponopono ʻana i ka hoʻoponopono ʻana i nā kiko.

ʻO ka hōʻike o ka papahana hoʻomākaukau PRP i ka noiʻi lapaʻau ʻaʻole kūlike loa.ʻO ka hapa nui o nā haʻawina i paʻi ʻia ʻaʻole i manaʻo i ke ʻano hoʻomākaukau PRP pono no ka hana hou ʻana o ka papahana.ʻAʻohe manaʻo ʻokoʻa ma waena o nā hōʻailona lapaʻau, no laila paʻakikī ke hoʻohālikelike i nā huahana PRP a me nā hopena lapaʻau pili.I ka hapanui o nā hihia i hōʻike ʻia, ua hoʻokaʻawale ʻia ka platelet concentration therapy ma lalo o ka huaʻōlelo "PRP", ʻoiai no ka hōʻailona hōʻailona like.No kekahi mau lāʻau lapaʻau (e like me ka OA a me ka tendinosis), ua holomua ka hoʻomaopopo ʻana i ka hoʻololi ʻana o ka hoʻomākaukau ʻana o ka PRP, nā ala lawe, ka hana platelet a me nā mea PRP ʻē aʻe e pili ana i ka hoʻoponopono ʻana o ka ʻiʻo a me ka hoʻōla hou ʻana o ke kino.Eia nō naʻe, pono ka noiʻi hou aʻe e hiki ai i ka ʻae ʻana i nā huaʻōlelo PRP e pili ana i nā mea ola ola PRP i mea e mālama pono ai i kekahi mau pathologies a me nā maʻi.

ʻO ke kūlana o ka ʻōnaehana hoʻonohonoho PRP

Hoʻopilikia ʻia ka hoʻohana ʻana i ka biotherapy PRP autologous e ka heterogeneity o ka hoʻomākaukau ʻana o PRP, ka inoa like ʻole a me ka hoʻomaʻamaʻa maikaʻi ʻole o nā alakaʻi e pili ana i nā hōʻike (ʻo ia hoʻi, he nui nā ʻano hoʻomākaukau no ka hana ʻana i nā hue lapaʻau lapaʻau).Hiki ke wānana ʻia he ʻano like ʻole ka ʻike o ka PRP piha, ka maʻemaʻe a me nā hiʻohiʻona olaola o PRP a me nā huahana pili, a pili i ka pono olaola a me nā hopena hoʻokolohua lapaʻau.ʻO ke koho ʻana o ka mea hoʻomākaukau PRP e hoʻolauna i ka ʻano kī mua.I loko o ka lāʻau lapaʻau hōʻano hou, hiki i nā mea hoʻomaʻamaʻa ke hoʻohana i ʻelua mau mea hoʻomākaukau a me nā ʻano hana hoʻomākaukau PRP.Hoʻohana ka hoʻomākaukau ʻana i kahi mea hoʻokaʻawale koko maʻamau, e hana ana i ke koko piha i hōʻiliʻili ʻia e ia iho.Hoʻohana kēia ʻano hana i ka pahu centrifuge kahe mau a i ʻole ka ʻenehana hoʻokaʻawale disk a me nā ʻanuʻu centrifuge paʻakikī a palupalu.Hoʻohana ʻia ka hapa nui o kēia mau mea hana i ke ʻoki.ʻO kekahi ala ʻē aʻe ka hoʻohana ʻana i ka ʻenehana centrifugal gravity a me nā mea hana.Hoʻohana ʻia ka centrifugation G-force kiʻekiʻe e hoʻokaʻawale i ka papa melemele o ESR mai ka ʻāpana koko i loaʻa nā platelets a me nā keʻokeʻo keʻokeʻo.ʻOi aku ka liʻiliʻi o kēia mau mea hoʻokalakupua ma mua o nā mea hoʻokaʻawale koko a hiki ke hoʻohana ʻia ma ka ʻaoʻao o kahi moe.Ma ka ʻokoʻa ģ - ʻO ka ikaika a me ka manawa centrifugation e alakaʻi i nā ʻokoʻa nui i ka hoʻoulu ʻana, ka noʻonoʻo ʻana, ka maʻemaʻe, ka hiki, a me ke kūlana hoʻoikaika o nā platelets kaʻawale.Hiki ke hoʻohana ʻia nā ʻano mea hoʻomākaukau PRP pāʻoihana i ka ʻāpana hope, e hopena i nā loli hou i ka ʻike huahana.

ʻO ka nele o ka ʻae ʻana i ke ʻano o ka hoʻomākaukau ʻana a me ka hōʻoia ʻana o ka PRP ke hoʻomau nei ke alakaʻi i ka like ʻole o ka mālama ʻana i ka PRP, a aia nā ʻokoʻa nui i ka hoʻomākaukau ʻana i ka PRP, ka maikaʻi o ka laʻana a me nā hopena lapaʻau.Ua hōʻoia ʻia a hoʻopaʻa inoa ʻia nā lako PRP pāʻoihana e like me nā kikoʻī o ka mea hana waiwai, e hoʻonā i nā ʻano like ʻole i waena o nā lako PRP i loaʻa i kēia manawa.

E hoʻomaopopo i ka nui o ka platelet in vitro a me in vivo

ʻO ka hopena therapeutic o PRP a me nā platelet concentrates ʻē aʻe mai ka hoʻokuʻu ʻana i nā mea like ʻole e pili ana i ka hoʻoponopono ʻana a me ka hana hou ʻana.Ma hope o ka ho'āla ʻia ʻana o nā platelets, e hana ʻia nā platelets thrombus platelet, kahi e lawelawe ai ma ke ʻano he extracellular matrix no ka hoʻolalelale ʻana i nā cell proliferation a me ka ʻokoʻa.No laila, kūpono ka manaʻo ʻana e alakaʻi ʻia ka nui o ka platelet i ke kiʻekiʻe o ka pae kūloko o nā mea bioactive platelet.Eia nō naʻe, ʻaʻole hiki ke hoʻopaʻa ʻia ka pilina ma waena o ka nui a me ka neʻe ʻana o nā platelets a me ka neʻe ʻana o ka platelet bioactive growth factor a me ka lāʻau lapaʻau, no ka mea, aia nā ʻokoʻa nui i ka helu platelet baseline ma waena o kēlā me kēia maʻi, a aia nā ʻokoʻa ma waena o nā ʻano hana hoʻomākaukau PRP.Pēlā nō, aia kekahi mau mea ulu platelet e pili ana i ka mīkini hoʻoponopono ʻiʻo i loko o ka ʻāpana plasma o PRP (no ka laʻana, ka mea ulu ate a me ka mea ulu like me ka insulin 1).No laila, ʻaʻole e pili ka nui o ka platelet i ka hiki ke hoʻoponopono i kēia mau mea ulu.

Ua kaulana loa ka noiʻi in vitro PRP no ka mea hiki ke hoʻomalu pono ʻia nā ʻāpana like ʻole o kēia mau haʻawina a hiki ke loaʻa koke nā hopena.Ua hōʻike ʻia kekahi mau haʻawina e pane ana nā cell i ka PRP ma ke ʻano hilinaʻi.Ua hōʻike ʻo Nguyen lāua ʻo Pham ʻaʻole kūpono nā ʻano kiʻekiʻe kiʻekiʻe o GF i ke kaʻina hana o ka hoʻoulu ʻana o ke kelepona, a he mea kūʻē paha ia.Ua hōʻike ʻia kekahi mau haʻawina in vitro e loaʻa paha nā hopena koʻikoʻi o ka PGF kiʻekiʻe.ʻO kekahi kumu ʻo ia ka helu palena o nā mea hoʻokipa membrane cell.No laila, ke kiʻekiʻe loa ka pae PGF i hoʻohālikelike ʻia me nā mea loaʻa i loaʻa, e loaʻa iā lākou kahi hopena maikaʻi ʻole i ka hana kelepona.

ʻO ke koʻikoʻi o ka ʻike platelet concentration in vitro

ʻOiai he nui nā pono o ka noiʻi in vitro, aia kekahi mau hemahema.In vitro, ma muli o ka hoʻomau mau ʻana ma waena o nā ʻano cell like ʻole i kekahi ʻiʻo ma muli o ke ʻano o ka ʻiʻo a me ka ʻiʻo cellular, paʻakikī ke hana hou i loko o ka vitro i loko o kahi ʻano moʻomeheu ʻelua.ʻO ka nui o ke kelepona e hiki ke hoʻopili i ke ala hōʻailona kelepona ma lalo o 1% o ke kūlana kiko.Kāohi ʻia nā ʻiʻo kīʻaha moʻomeheu ʻelua i ka ʻike ʻia ʻana o nā cell i ka extracellular matrix (ECM).Eia kekahi, ʻo ka ʻenehana moʻomeheu maʻamau e alakaʻi i ka hōʻiliʻili ʻana o ka ʻōpala cell a me ka hoʻohana mau ʻana i ka meaʻai.No laila, ʻokoʻa ka moʻomeheu in vitro mai kahi kūlana paʻa, hāʻawi ʻana i ka oxygen tissue a i ʻole ka hoʻololi koke ʻana o ka moʻomeheu moʻomeheu, a ua paʻi ʻia nā hopena kū'ē, e hoʻohālikelike ana i ka hopena lapaʻau o PRP me ka noiʻi in vitro o nā cell kikoʻī, nā ʻano kiko a me nā platelet. nā manaʻo.ʻO Graziani a me nā mea ʻē aʻe.Uaʻikeʻia i loko o ka vitro,ʻo ka hopena nui loa i ka hoʻonuiʻana o nā osteoblasts a me nā fibroblasts i loaʻa i ka PRP platelet concentration 2.5 manawa kiʻekiʻe ma mua o ka helu kumu.I ka hoʻokaʻawale ʻana, ua hōʻike ʻia nā ʻikepili lapaʻau i hāʻawi ʻia e Park a me nā hoahana ma hope o ka hui ʻana o ka spinal, pono e hoʻonui ʻia ka pae platelet PRP ma mua o 5 mau manawa ma mua o ke kumu kumu e hoʻoulu ai i nā hopena maikaʻi.Ua hōʻike pū ʻia nā hopena like ʻole ma waena o ka ʻikepili proliferation tendon in vitro a me nā hopena lapaʻau.

（Paʻi hou ʻia nā mea o kēia ʻatikala, ʻaʻole mākou e hāʻawi i ka hōʻoiaʻiʻo a i ʻole ka hōʻoia ʻana no ka pololei, hilinaʻi a i ʻole ka piha o nā mea i loko o kēia ʻatikala, a ʻaʻole kuleana no nā manaʻo o kēia ʻatikala, e ʻoluʻolu e hoʻomaopopo.)